To investigate the effect of different concentrations of alfalfa and Bermuda grass extracts on callus induction and rye embryo regeneration, the regeneration and growth of rye seedlings were randomly carried out at the Islamic Azad University, Tabriz branch in 2012, using a factorial experiment based on completely randomized design in 3 replicates in both greenhouse and in-vitro conditions. In this study, immature rye embryos were used as explants for generating callus, and water extracts of alfalfa and Bermuda grass were added to the environment with a concentration of 5, 10, 15, 20, 25 and 30 percent. This extract was also used in the regeneration period. The results showed that callus induction from Rye and its components in 5% alfalfa extracts significantly increased compared to the control condition, but increasing alfalfa extract concentration caused a significant reduction in these traits. The use of 25 and 30% alfalfa extracts stopped callus induction and its components. The application of bermuda grass extract with a concentration of 30 %, reduced rye callus induction to 50 percent. Alfalfa extract concentrations of 20 and 25 percent, respectively caused a reduction of 35 and 75 percent in callus fresh weight in comparison with the control conditions. Applying 25 and 30 percent concentrations of bermuda grass extract led to a reduction of 68 and 80% respectively. Alfalfa extracts with 15, 20 and 25 percent concentrations caused reductions of 40, 63 and 69% in rooting. The 30% concentration of alfalfa extract stopped rooting in rye callus. Concentrations of 15, 20, 25 and 30% caused reductions of 31, 50, 55 and 81 percent in the shooting of rye callus, respectively. The results indicate that alfalfa extract had greater effect on rooting, and the bermuda grass extracts had greater effect on shooting.